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Your current location:Home / News / Industry trendsWhat are the low temperature storage equipment in the laboratory?
Article source:What are the low temperature storage equipment in the laboratory? Hits:520 Release time:2017-11-13
Today, I want to share with you the content about low temperature storage in the laboratory, hoping to help you with your daily sales work.
The importance of low temperature storage in laboratory
What items need low temperature preservation?
1. Most medical reagents, biological agents, enzymes, vaccines and drugs.
2. Bacteria, viruses or other animal and plant samples, as well as some special cells, skin, organs or other living samples that need to be preserved or studied by hospitals and scientific research institutions.
3. Genes or semen of excellent varieties preserved in animal husbandry system and aquatic agriculture.
4. The chemical industry needs low temperature treatment for some electronic components or precision instruments.
5. Standard solution, reference material, etc. of general laboratory, bacteria and culture medium of microorganism room.
What is the significance of cryopreservation?
1. At low temperature, some drugs, biological agents, enzyme vaccines, etc. should be prevented from deterioration and failure due to the influence of external environment. Low temperature can slow down the denaturation or degradation of active ingredients.
2. In vivo, low temperature can slow down cell division and metabolism, keep good original state for a long time, and greatly improve the survival rate.
2Laboratory low temperature storage equipment
Classification of low temperature storage equipment
（1） Medicine cool cabinet / medicine cold box
（2） Blood refrigerator
（3） Low temperature refrigerator / ultra low temperature refrigerator
（4） Liquid nitrogen tank
A variety of factors are essential to ensure the quality of drugs, samples and reagents, including storage temperature, expiration date, UV exposure and humidity.
The most important is storage temperature. Generally, the storage temperature of drugs we see includes normal temperature, shade and cold storage. The specific temperature required by these conditions are:
Normal temperature: 10 ℃ ~ 30 ℃
Cool storage temperature: no more than 20 ℃
Cold storage temperature: 2 ℃ ~ 10 ℃
If the temperature requirement is not specified, it generally refers to normal temperature.
The relative humidity should be between 35% and 75%.
Medicine shade cabinet (8 ℃ ~ 20 ℃)
It is the necessary cool medicine storage equipment for all drugstores, suitable for storing medicine, reagents, samples, etc. at 8 ℃ ~ 20 ℃.
The main medicines stored in the medicine shade cabinet are:
(1) Cardiovascular and cerebrovascular drugs;
(2) Antibiotics;
(3) Eye drops and other liquid drugs;
(4) Suppository pastes that may soften above 20 ℃;
(5) Medication for children;
(6) Vitamins and other nutritional drugs.
Medicine refrigerator (2 ℃ ~ 8 ℃)
It is a special cold storage equipment for refrigerated drugs in the medical industry, and can also be used for the refrigeration of biological products, reagents, vaccines, etc. It is suitable for hospitals, pharmacies, clinics, centers for disease control, universities, blood stations, inspection and quarantine institutions and pharmaceutical factories, etc.
Drug freezer is mainly used for cold storage of vaccines, blood products, monoclonal antibodies, insulin, interferon, some antibiotics, other protein preparations, etc.
The difference between medicine refrigerator and household refrigerator
Medicine shade cabinet (8 ℃ ~ 20 ℃)
It is the necessary cool medicine storage equipment for all drugstores, suitable for storing medicine, reagents, samples, etc. at 8 ℃ ~ 20 ℃.
The main medicines stored in the medicine shade cabinet are:
(1) Cardiovascular and cerebrovascular drugs;
(2) Antibiotics;
(3) Eye drops and other liquid drugs;
(4) Suppository pastes that may soften above 20 ℃;
(5) Medication for children;
(6) Vitamins and other nutritional drugs.
Medicine refrigerator (2 ℃ ~ 8 ℃)
It is a special cold storage equipment for refrigerated drugs in the medical industry, and can also be used for the refrigeration of biological products, reagents, vaccines, etc. It is suitable for hospitals, pharmacies, clinics, centers for disease control, universities, blood stations, inspection and quarantine institutions and pharmaceutical factories, etc.
Drug freezer is mainly used for cold storage of vaccines, blood products, monoclonal antibodies, insulin, interferon, some antibiotics, other protein preparations, etc.
The difference between medicine refrigerator and household refrigerator
类别 | 家用(yòng)冰箱 | 药品冷藏箱 | 药品冷藏箱优势 |
温度范围 | 0-10℃ | 2-8℃ | 避免出现0℃冰点以下，防止物(wù)品冻结 |
温度控制 | 机械温度控制 | 数字温控制 | 控温精确，不受环境温度影响，避免物(wù)品损坏的风险 |
制冷方式 | 直冷式 | 风冷式 | 循环风机及风道设计，保证冷气循环，避免冰点导致物(wù)品损坏 |
报警提示 | 无报警 | 有(yǒu)报警 | 多(duō)重故障报警，灯光闪烁报警方式，可(kě)接遠(yuǎn)程报警 |
关键部件 | 成本控制要求严 | 高成本國(guó)际名牌 | 避免低成本部件对专业性能(néng)的影响 |
存放用(yòng)途 | 存放食品蔬菜饮料 | 存放试剂疫苗血液 | 专业的存放设备，标准高，存储放心 |
存放时间 | 存放时间要求不高 | 存放时间要求不高 | 存放时间选择余地大，不用(yòng)担心短期存放的风险 |
Blood refrigerator is a professional cold storage equipment for the medical industry to refrigerate blood (whole blood). It is suitable for hospitals, blood stations and medical research departments to store blood.
Low temperature refrigerator is a kind of freezer which can obtain a certain temperature by mechanical refrigeration. According to the temperature, it can be divided into low temperature refrigerator and ultra-low temperature refrigerator.
-25 ℃ refrigerator
-40 ℃ low temperature refrigerator
-60 ℃ low temperature refrigerator
-86 ℃ refrigerator
-105 ℃ low temperature refrigerator
-136 ℃ low temperature refrigerator
-152 ℃ low temperature refrigerator
Use of low temperature refrigerator
It is mainly used in blood station, hospital, health and epidemic prevention system, scientific research institute, electronic industry, chemical industry, bioengineering, University Laboratory, military enterprise and ocean fishery company.
(1) Food freezing: temperature range - 18 ℃ ~ - 28 ℃, used for meat, fish, seafood and other cold storage, storage period of 1-3 months; cold food and catering industry
(2) Scientific research: the temperature range is - 30 ℃ ~ - 85 ℃, which is used for the test of performance change of instrument products and electronic components in low temperature environment;
(3) Biomedical Engineering: temperature range - 30 ℃ ~ - 85 ℃ (or lower), used to store organs, skin, bacteria, viruses, sperm, blood, and other biological products.
As for the contents of liquid nitrogen tank, we will also sort out very comprehensive contents:
Purpose:
1. Animal husbandry: frozen storage of livestock semen and embryos;
2. Medical industry: cryopreservation of human stem cells, plasma, internal organs, biological samples and treatment of skin diseases;
3. Scientific research institutions: incubators and storage equipment for vaccines, viruses and other microorganisms;
4. Industrial field: deep cooling treatment of tools, cutting tools, cutting tools, mechanical cold assembly, etc.
5. Food: quick frozen food, liquid nitrogen ice cream, molecular food, beverage processing, etc.
Liquid nitrogen tank classification:
1. Portable liquid nitrogen tank
It is mainly used for breeding of frozen semen and portable transportation of biological samples;
2. Storage liquid nitrogen tank
It is mainly used for long-term static preservation of biological samples;
3. Transport liquid nitrogen tank
For long-distance transportation of liquid nitrogen or biological samples;
4. Large diameter laboratory liquid nitrogen tank
It is mainly used for long-term preservation of biological samples but needs to extract samples frequently;
5. Air transport liquid nitrogen tank
It is specially used to transport biological samples through aircraft and other aircrafts.
Advantages and disadvantages of ultra-low temperature refrigerators and liquid nitrogen tanks for sample storage
Cryogenics and liquid nitrogen tanks can be used for cryopreservation of biological samples such as stem cells, plasma, semen, cells and strains. Their differences are as follows:
1. The temperature of liquid nitrogen is lower, which can reach - 196 ℃. The life activity in the sample basically stops at this temperature, and the stability of the sample can be preserved for a long time.
2. Ultra low temperature refrigerator is electric refrigeration, the cooling speed of electric refrigeration is lower than liquid nitrogen, once the container is opened to take and place samples, it is easy to cause a wide range of temperature fluctuations, and the temperature recovery time is relatively long, so it is more suitable for the case of less opening to take and place samples. In addition, the electric refrigerator must guarantee the power supply. In case of power failure, liquid nitrogen equipment is recommended to provide backup storage.
3. It is necessary to further protect the cross contamination of samples in liquid-phase storage of liquid nitrogen containers. It is easy for liquid nitrogen to permeate into the cryopreservation tube due to the poor sealing of the cryopreservation tube, which increases the risk of cross contamination between samples. One of the solutions is to use a special cryopreservation tube sleeve to heat shrink seal each cryopreservation tube, or use a sealing film to wrap 2-3 circles at the interface between the cryopreservation tube cap and the tube body for storage.
1Types of samples preserved at low temperature in the laboratory
血液
(1)血液组成
(2) Blood preservation
1. Whole blood and red blood cells: (blood refrigerator)
Storage temperature: 2 ℃ ~ 6 ℃.
2. Frozen red blood cells:
Storage temperature: the frozen red blood cells containing 20% glycerol are stored below - 120 ℃, and the frozen red blood cells containing 40% glycerol are stored below - 65 ℃.
3. Platelets, granulocytes (platelet shaker)
Storage temperature: 22 ℃± 2 ℃, oscillation culture
4. Plasma: (low temperature refrigerator, liquid nitrogen tank)
Storage temperature: below - 18 ℃.
After thawing, it should be stored at 2 ℃ ~ 6 ℃ and infused within 24 hours
Drugs
Drugs refer to substances used to prevent, treat and diagnose human diseases, purposefully regulate human physiological functions and stipulate indications or functions, indications, usage and dosage, including Chinese herbal medicines, Chinese herbal pieces, Chinese patent medicines, chemical raw materials and their preparations, antibiotics, biochemical drugs, radioactive drugs, serum, vaccines, blood products and diagnostic drugs, etc
Maintenance of the common variable quality dosage forms in drugs
1) Tablet, syrup, capsule: 8 ~ 20 ℃; (medicine shade cabinet)
2) Water preparation, ointment, suppository, water injection and powder injection: normal temperature 10-30 ℃
3) Antibiotics, biological products, enzyme preparations and other injections: 2-8 ℃ (drug refrigerator)
vaccine
Vaccine refers to the biological products produced by various pathogenic microorganisms for vaccination. The vaccine made of bacteria or spirochetes is also called vaccine. There are two kinds of vaccines: live vaccine and dead vaccine. Commonly used live vaccines include BCG vaccine, polio vaccine, measles vaccine, plague vaccine, etc. The commonly used dead vaccines are pertussis vaccine, typhoid vaccine, meningococcal vaccine, cholera vaccine, etc.
Vaccine storage temperature:
Live vaccine: below - 15 ℃ (low temperature refrigerator)
Recommendation: Aucma low temperature refrigerator
Inactivated vaccine (dead vaccine): 2-8 ℃ (drug refrigerator)
Recommendation: Aucma medical refrigerator
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